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the primary human renal podocytes  (Thermo Fisher)


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    Thermo Fisher the primary human renal podocytes
    The crosstalk effect among HK-2 cells, THP-1 cells, and primary human renal <t>podocytes</t> was investigated as follows: (A) the serum from DKD mice (10%) was added to HK-2 cells for culture for 24 h, after which relevant indicators were detected. The supernatant from HK-2 cells was then transferred to THP-1 cells to assess the functional changes in THP-1 cells, and subsequently, the THP-1 cell supernatant was added to primary human renal podocytes to observe their functional alterations. (B) The levels of IGFBP2 and IGFBP4 following HK-2 cell stimulation were measured. (C) The polarization of THP-1 cells was assessed using flow cytometry, where CD86% served as the marker for M1 polarization and CD206% indicated M2 polarization. (D) ELISA was employed to detect changes in complement proteins C3, C4B, C5, and C9 in THP-1 cells. (E) The levels of MAC and MBL in THP-1 cells were also measured using ELISA. (F) Podocyte proliferation was evaluated using the CCK-8 assay. (G) Apoptosis of primary human renal podocytes was analyzed through flow cytometry. (H) The expression of reactive oxygen species (ROS) in primary human renal podocytes was visualized by immunofluorescence, with green fluorescence indicating the intensity of ROS expression. Data are presented as mean ± SD ( n = 3; * p < .05, ** p < .01, *** p < .001).
    The Primary Human Renal Podocytes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/the primary human renal podocytes/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    the primary human renal podocytes - by Bioz Stars, 2026-03
    90/100 stars

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    1) Product Images from "IGFBP2 and IGFBP4 interact to activate complement pathway in diabetic kidney disease"

    Article Title: IGFBP2 and IGFBP4 interact to activate complement pathway in diabetic kidney disease

    Journal: Renal Failure

    doi: 10.1080/0886022X.2024.2440528

    The crosstalk effect among HK-2 cells, THP-1 cells, and primary human renal podocytes was investigated as follows: (A) the serum from DKD mice (10%) was added to HK-2 cells for culture for 24 h, after which relevant indicators were detected. The supernatant from HK-2 cells was then transferred to THP-1 cells to assess the functional changes in THP-1 cells, and subsequently, the THP-1 cell supernatant was added to primary human renal podocytes to observe their functional alterations. (B) The levels of IGFBP2 and IGFBP4 following HK-2 cell stimulation were measured. (C) The polarization of THP-1 cells was assessed using flow cytometry, where CD86% served as the marker for M1 polarization and CD206% indicated M2 polarization. (D) ELISA was employed to detect changes in complement proteins C3, C4B, C5, and C9 in THP-1 cells. (E) The levels of MAC and MBL in THP-1 cells were also measured using ELISA. (F) Podocyte proliferation was evaluated using the CCK-8 assay. (G) Apoptosis of primary human renal podocytes was analyzed through flow cytometry. (H) The expression of reactive oxygen species (ROS) in primary human renal podocytes was visualized by immunofluorescence, with green fluorescence indicating the intensity of ROS expression. Data are presented as mean ± SD ( n = 3; * p < .05, ** p < .01, *** p < .001).
    Figure Legend Snippet: The crosstalk effect among HK-2 cells, THP-1 cells, and primary human renal podocytes was investigated as follows: (A) the serum from DKD mice (10%) was added to HK-2 cells for culture for 24 h, after which relevant indicators were detected. The supernatant from HK-2 cells was then transferred to THP-1 cells to assess the functional changes in THP-1 cells, and subsequently, the THP-1 cell supernatant was added to primary human renal podocytes to observe their functional alterations. (B) The levels of IGFBP2 and IGFBP4 following HK-2 cell stimulation were measured. (C) The polarization of THP-1 cells was assessed using flow cytometry, where CD86% served as the marker for M1 polarization and CD206% indicated M2 polarization. (D) ELISA was employed to detect changes in complement proteins C3, C4B, C5, and C9 in THP-1 cells. (E) The levels of MAC and MBL in THP-1 cells were also measured using ELISA. (F) Podocyte proliferation was evaluated using the CCK-8 assay. (G) Apoptosis of primary human renal podocytes was analyzed through flow cytometry. (H) The expression of reactive oxygen species (ROS) in primary human renal podocytes was visualized by immunofluorescence, with green fluorescence indicating the intensity of ROS expression. Data are presented as mean ± SD ( n = 3; * p < .05, ** p < .01, *** p < .001).

    Techniques Used: Functional Assay, Cell Stimulation, Flow Cytometry, Marker, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Expressing, Immunofluorescence, Fluorescence

    Effects of exogenous addition of IGFBP2 and IGFBP4 on THP-1 cells and primary human renal podocytes. (A) Following the addition of recombinant IGFBP2 and IGFBP4 proteins to THP-1 cells, the supernatant was collected for the assessment of related indices, which were subsequently applied to primary human renal podocytes to observe functional changes. (B) Different concentrations of recombinant IGFBP2 and IGFBP4 (50 ng/mL, 100 ng/mL, 200 ng/mL, and 400 ng/mL) were found to stimulate the levels of complement proteins C3, C4B, C5, C9, as well as MAC and MBL in THP-1 cells. (C) The effects of individually adding IGFBP2h or IGFBP4 recombinant protein, or their combined addition, on the levels of complement proteins C3, C4B, C5, C9, MAC, and MBL in THP-1 cells were assessed. (D) Flow cytometry analysis was conducted to determine the impact of IGFBP2h or IGFBP4 recombinant protein, either alone or in combination, on the polarization of THP-1 cells. (E) The influence of THP-1 cell supernatant on podocyte proliferation was evaluated using the CCK-8 assay. (F) The effect of THP-1 cell supernatant on podocyte apoptosis was measured through flow cytometry. (G) Immunofluorescence was utilized to observe the effect of THP-1 cell supernatant on ROS expression in primary human renal podocytes. (H) The impact of THP-1 cell supernatant on podocyte morphology was also assessed. Data are presented as mean ± SD. n = 3; * p < .05, ** p < .01, *** p < .001.
    Figure Legend Snippet: Effects of exogenous addition of IGFBP2 and IGFBP4 on THP-1 cells and primary human renal podocytes. (A) Following the addition of recombinant IGFBP2 and IGFBP4 proteins to THP-1 cells, the supernatant was collected for the assessment of related indices, which were subsequently applied to primary human renal podocytes to observe functional changes. (B) Different concentrations of recombinant IGFBP2 and IGFBP4 (50 ng/mL, 100 ng/mL, 200 ng/mL, and 400 ng/mL) were found to stimulate the levels of complement proteins C3, C4B, C5, C9, as well as MAC and MBL in THP-1 cells. (C) The effects of individually adding IGFBP2h or IGFBP4 recombinant protein, or their combined addition, on the levels of complement proteins C3, C4B, C5, C9, MAC, and MBL in THP-1 cells were assessed. (D) Flow cytometry analysis was conducted to determine the impact of IGFBP2h or IGFBP4 recombinant protein, either alone or in combination, on the polarization of THP-1 cells. (E) The influence of THP-1 cell supernatant on podocyte proliferation was evaluated using the CCK-8 assay. (F) The effect of THP-1 cell supernatant on podocyte apoptosis was measured through flow cytometry. (G) Immunofluorescence was utilized to observe the effect of THP-1 cell supernatant on ROS expression in primary human renal podocytes. (H) The impact of THP-1 cell supernatant on podocyte morphology was also assessed. Data are presented as mean ± SD. n = 3; * p < .05, ** p < .01, *** p < .001.

    Techniques Used: Recombinant, Functional Assay, Flow Cytometry, CCK-8 Assay, Immunofluorescence, Expressing



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    the primary human renal podocytes  (Thermo Fisher)
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    Thermo Fisher the primary human renal podocytes
    The crosstalk effect among HK-2 cells, THP-1 cells, and primary human renal <t>podocytes</t> was investigated as follows: (A) the serum from DKD mice (10%) was added to HK-2 cells for culture for 24 h, after which relevant indicators were detected. The supernatant from HK-2 cells was then transferred to THP-1 cells to assess the functional changes in THP-1 cells, and subsequently, the THP-1 cell supernatant was added to primary human renal podocytes to observe their functional alterations. (B) The levels of IGFBP2 and IGFBP4 following HK-2 cell stimulation were measured. (C) The polarization of THP-1 cells was assessed using flow cytometry, where CD86% served as the marker for M1 polarization and CD206% indicated M2 polarization. (D) ELISA was employed to detect changes in complement proteins C3, C4B, C5, and C9 in THP-1 cells. (E) The levels of MAC and MBL in THP-1 cells were also measured using ELISA. (F) Podocyte proliferation was evaluated using the CCK-8 assay. (G) Apoptosis of primary human renal podocytes was analyzed through flow cytometry. (H) The expression of reactive oxygen species (ROS) in primary human renal podocytes was visualized by immunofluorescence, with green fluorescence indicating the intensity of ROS expression. Data are presented as mean ± SD ( n = 3; * p < .05, ** p < .01, *** p < .001).
    The Primary Human Renal Podocytes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/the primary human renal podocytes/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    the primary human renal podocytes - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    primary human renal podocytes  (Thermo Fisher)
    90
    Thermo Fisher primary human renal podocytes
    The crosstalk effect among HK-2 cells, THP-1 cells, and primary human renal <t>podocytes</t> was investigated as follows: (A) the serum from DKD mice (10%) was added to HK-2 cells for culture for 24 h, after which relevant indicators were detected. The supernatant from HK-2 cells was then transferred to THP-1 cells to assess the functional changes in THP-1 cells, and subsequently, the THP-1 cell supernatant was added to primary human renal podocytes to observe their functional alterations. (B) The levels of IGFBP2 and IGFBP4 following HK-2 cell stimulation were measured. (C) The polarization of THP-1 cells was assessed using flow cytometry, where CD86% served as the marker for M1 polarization and CD206% indicated M2 polarization. (D) ELISA was employed to detect changes in complement proteins C3, C4B, C5, and C9 in THP-1 cells. (E) The levels of MAC and MBL in THP-1 cells were also measured using ELISA. (F) Podocyte proliferation was evaluated using the CCK-8 assay. (G) Apoptosis of primary human renal podocytes was analyzed through flow cytometry. (H) The expression of reactive oxygen species (ROS) in primary human renal podocytes was visualized by immunofluorescence, with green fluorescence indicating the intensity of ROS expression. Data are presented as mean ± SD ( n = 3; * p < .05, ** p < .01, *** p < .001).
    Primary Human Renal Podocytes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary human renal podocytes/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    primary human renal podocytes - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The crosstalk effect among HK-2 cells, THP-1 cells, and primary human renal podocytes was investigated as follows: (A) the serum from DKD mice (10%) was added to HK-2 cells for culture for 24 h, after which relevant indicators were detected. The supernatant from HK-2 cells was then transferred to THP-1 cells to assess the functional changes in THP-1 cells, and subsequently, the THP-1 cell supernatant was added to primary human renal podocytes to observe their functional alterations. (B) The levels of IGFBP2 and IGFBP4 following HK-2 cell stimulation were measured. (C) The polarization of THP-1 cells was assessed using flow cytometry, where CD86% served as the marker for M1 polarization and CD206% indicated M2 polarization. (D) ELISA was employed to detect changes in complement proteins C3, C4B, C5, and C9 in THP-1 cells. (E) The levels of MAC and MBL in THP-1 cells were also measured using ELISA. (F) Podocyte proliferation was evaluated using the CCK-8 assay. (G) Apoptosis of primary human renal podocytes was analyzed through flow cytometry. (H) The expression of reactive oxygen species (ROS) in primary human renal podocytes was visualized by immunofluorescence, with green fluorescence indicating the intensity of ROS expression. Data are presented as mean ± SD ( n = 3; * p < .05, ** p < .01, *** p < .001).

    Journal: Renal Failure

    Article Title: IGFBP2 and IGFBP4 interact to activate complement pathway in diabetic kidney disease

    doi: 10.1080/0886022X.2024.2440528

    Figure Lengend Snippet: The crosstalk effect among HK-2 cells, THP-1 cells, and primary human renal podocytes was investigated as follows: (A) the serum from DKD mice (10%) was added to HK-2 cells for culture for 24 h, after which relevant indicators were detected. The supernatant from HK-2 cells was then transferred to THP-1 cells to assess the functional changes in THP-1 cells, and subsequently, the THP-1 cell supernatant was added to primary human renal podocytes to observe their functional alterations. (B) The levels of IGFBP2 and IGFBP4 following HK-2 cell stimulation were measured. (C) The polarization of THP-1 cells was assessed using flow cytometry, where CD86% served as the marker for M1 polarization and CD206% indicated M2 polarization. (D) ELISA was employed to detect changes in complement proteins C3, C4B, C5, and C9 in THP-1 cells. (E) The levels of MAC and MBL in THP-1 cells were also measured using ELISA. (F) Podocyte proliferation was evaluated using the CCK-8 assay. (G) Apoptosis of primary human renal podocytes was analyzed through flow cytometry. (H) The expression of reactive oxygen species (ROS) in primary human renal podocytes was visualized by immunofluorescence, with green fluorescence indicating the intensity of ROS expression. Data are presented as mean ± SD ( n = 3; * p < .05, ** p < .01, *** p < .001).

    Article Snippet: The primary human renal podocytes were purchased from Wuhan Punosay Life Technology Co., Ltd. (CP-H075) (Wuhan, China).

    Techniques: Functional Assay, Cell Stimulation, Flow Cytometry, Marker, Enzyme-linked Immunosorbent Assay, CCK-8 Assay, Expressing, Immunofluorescence, Fluorescence

    Effects of exogenous addition of IGFBP2 and IGFBP4 on THP-1 cells and primary human renal podocytes. (A) Following the addition of recombinant IGFBP2 and IGFBP4 proteins to THP-1 cells, the supernatant was collected for the assessment of related indices, which were subsequently applied to primary human renal podocytes to observe functional changes. (B) Different concentrations of recombinant IGFBP2 and IGFBP4 (50 ng/mL, 100 ng/mL, 200 ng/mL, and 400 ng/mL) were found to stimulate the levels of complement proteins C3, C4B, C5, C9, as well as MAC and MBL in THP-1 cells. (C) The effects of individually adding IGFBP2h or IGFBP4 recombinant protein, or their combined addition, on the levels of complement proteins C3, C4B, C5, C9, MAC, and MBL in THP-1 cells were assessed. (D) Flow cytometry analysis was conducted to determine the impact of IGFBP2h or IGFBP4 recombinant protein, either alone or in combination, on the polarization of THP-1 cells. (E) The influence of THP-1 cell supernatant on podocyte proliferation was evaluated using the CCK-8 assay. (F) The effect of THP-1 cell supernatant on podocyte apoptosis was measured through flow cytometry. (G) Immunofluorescence was utilized to observe the effect of THP-1 cell supernatant on ROS expression in primary human renal podocytes. (H) The impact of THP-1 cell supernatant on podocyte morphology was also assessed. Data are presented as mean ± SD. n = 3; * p < .05, ** p < .01, *** p < .001.

    Journal: Renal Failure

    Article Title: IGFBP2 and IGFBP4 interact to activate complement pathway in diabetic kidney disease

    doi: 10.1080/0886022X.2024.2440528

    Figure Lengend Snippet: Effects of exogenous addition of IGFBP2 and IGFBP4 on THP-1 cells and primary human renal podocytes. (A) Following the addition of recombinant IGFBP2 and IGFBP4 proteins to THP-1 cells, the supernatant was collected for the assessment of related indices, which were subsequently applied to primary human renal podocytes to observe functional changes. (B) Different concentrations of recombinant IGFBP2 and IGFBP4 (50 ng/mL, 100 ng/mL, 200 ng/mL, and 400 ng/mL) were found to stimulate the levels of complement proteins C3, C4B, C5, C9, as well as MAC and MBL in THP-1 cells. (C) The effects of individually adding IGFBP2h or IGFBP4 recombinant protein, or their combined addition, on the levels of complement proteins C3, C4B, C5, C9, MAC, and MBL in THP-1 cells were assessed. (D) Flow cytometry analysis was conducted to determine the impact of IGFBP2h or IGFBP4 recombinant protein, either alone or in combination, on the polarization of THP-1 cells. (E) The influence of THP-1 cell supernatant on podocyte proliferation was evaluated using the CCK-8 assay. (F) The effect of THP-1 cell supernatant on podocyte apoptosis was measured through flow cytometry. (G) Immunofluorescence was utilized to observe the effect of THP-1 cell supernatant on ROS expression in primary human renal podocytes. (H) The impact of THP-1 cell supernatant on podocyte morphology was also assessed. Data are presented as mean ± SD. n = 3; * p < .05, ** p < .01, *** p < .001.

    Article Snippet: The primary human renal podocytes were purchased from Wuhan Punosay Life Technology Co., Ltd. (CP-H075) (Wuhan, China).

    Techniques: Recombinant, Functional Assay, Flow Cytometry, CCK-8 Assay, Immunofluorescence, Expressing